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Issue 25, 2017
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Intracellular synthesis of D-aminoluciferin for bioluminescence generation

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D-Luciferin is the most widely used substrate for bioluminescence (BL) applications but its low chemical stability always affects its performance. Herein, we rationally designed two chemically stable precursor molecules CBT-D-cystine-CBT (D-1) and CBT-L-cystine-CBT (L-1), and subjected them to reduction-controlled condensation to form 1-oligomer and subsequent proteolysis to yield D-aminoluciferin for BL generation in cells and in vivo. We envision that our precursor molecules might serve as D-luciferin alternatives for a wide range of BL applications in the near future.

Graphical abstract: Intracellular synthesis of d-aminoluciferin for bioluminescence generation

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The article was received on 08 Feb 2017, accepted on 07 Mar 2017 and first published on 07 Mar 2017

Article type: Communication
DOI: 10.1039/C7CC00999B
Citation: Chem. Commun., 2017,53, 3567-3570
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    Intracellular synthesis of D-aminoluciferin for bioluminescence generation

    Z. Zheng, G. Li, C. Wu, M. Zhang, Y. Zhao and G. Liang, Chem. Commun., 2017, 53, 3567
    DOI: 10.1039/C7CC00999B

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