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Issue 27, 2017
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Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

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Abstract

We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase (TDG) activity through cyclic enzymatic repairing-mediated dual-signal amplification. This method exhibits excellent sensitivity with a detection limit of 5.6 × 10−7 U μL−1, and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells.

Graphical abstract: Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

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Publication details

The article was received on 06 Feb 2017, accepted on 13 Mar 2017, published on 13 Mar 2017 and first published online on 13 Mar 2017


Article type: Communication
DOI: 10.1039/C7CC00946A
Citation: Chem. Commun., 2017,53, 3878-3881
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    Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

    L. Wang, Z. Wang, Q. Zhang, B. Tang and C. Zhang, Chem. Commun., 2017, 53, 3878
    DOI: 10.1039/C7CC00946A

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