Issue 77, 2017

Single tube gene synthesis by phosphoramidate chemical ligation

Abstract

Templated chemical ligation of 5′-amino and 3′-phosphate oligonucleotides was used to synthesise a 762 base pair gene for green fluorescent protein. The phosphoramidate linkage can be read by DNA polymerase and transcribed to make RNA. We also show that phosphoramidate ligation and orthogonal CuAAC-mediated DNA ligation can be used simultaneously.

Graphical abstract: Single tube gene synthesis by phosphoramidate chemical ligation

Supplementary files

Article information

Article type
Communication
Submitted
01 Feb 2017
Accepted
26 Jun 2017
First published
15 Sep 2017

Chem. Commun., 2017,53, 10700-10702

Single tube gene synthesis by phosphoramidate chemical ligation

A. H. El-Sagheer and T. Brown, Chem. Commun., 2017, 53, 10700 DOI: 10.1039/C7CC00858A

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