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Issue 77, 2017
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Single tube gene synthesis by phosphoramidate chemical ligation

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Abstract

Templated chemical ligation of 5′-amino and 3′-phosphate oligonucleotides was used to synthesise a 762 base pair gene for green fluorescent protein. The phosphoramidate linkage can be read by DNA polymerase and transcribed to make RNA. We also show that phosphoramidate ligation and orthogonal CuAAC-mediated DNA ligation can be used simultaneously.

Graphical abstract: Single tube gene synthesis by phosphoramidate chemical ligation

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Publication details

The article was received on 01 Feb 2017, accepted on 26 Jun 2017 and first published on 15 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC00858A
Citation: Chem. Commun., 2017,53, 10700-10702
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    Single tube gene synthesis by phosphoramidate chemical ligation

    A. H. El-Sagheer and T. Brown, Chem. Commun., 2017, 53, 10700
    DOI: 10.1039/C7CC00858A

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