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A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

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Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterizing irreversible inhibition.

Graphical abstract: A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

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Publication details

The article was received on 18 Jan 2017, accepted on 17 Mar 2017 and first published on 17 Mar 2017


Article type: Communication
DOI: 10.1039/C7CC00476A
Citation: Chem. Commun., 2017, Advance Article
  • Open access: Creative Commons BY license
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    A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

    M. Yevglevskis, G. L. Lee, A. Nathubhai, Y. D. Petrova, T. D. James, M. D. Threadgill, T. J. Woodman and M. D. Lloyd, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC00476A

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