Mimicking Multivalent Protein-Carbohydrate Interaction for Monitoring Glucosamine Level in Biological Fluids and Pharmaceutical Tablet
A series of easily synthesizable ESIPT probes for dual mode sensing of glucosamine in water. A rapid change in color from bright yellow to colorless with prominent emission quenching was observed exclusively in presence of glucosamine at pH 7.0. The mechanistic investigation confirmed that the addition of D-(+)-glucosamine induced a shift in the keto-enol equilibrium of compound towards the enol side through multipoint hydrogen bonding and CH-pi interactions. Then the estimation of glucosamine was achieved in blood serum samples as well as in oral supplements. Finally, the portable low-cost color-strips have been developed for rapid on-site detection of glucosamine without involving any sophisticated instrument.