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Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

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Abstract

We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

Graphical abstract: Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

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Publication details

The article was received on 14 Dec 2016, accepted on 08 May 2017 and first published on 09 May 2017


Article type: Communication
DOI: 10.1039/C6CC09948C
Citation: Chem. Commun., 2017, Advance Article
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    Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

    J. A. González-Vera, D. Bouzada, C. Bouclier, M. Eugenio Vázquez and M. C. Morris, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C6CC09948C

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