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Issue 19, 2017
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Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

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Abstract

We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.

Graphical abstract: Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

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Publication details

The article was received on 14 Dec 2016, accepted on 13 Feb 2017 and first published on 13 Feb 2017


Article type: Communication
DOI: 10.1039/C6CC09916E
Citation: Chem. Commun., 2017,53, 2874-2877
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    Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

    H. Soleimaninejad, M. Z. Chen, X. Lou, T. A. Smith and Y. Hong, Chem. Commun., 2017, 53, 2874
    DOI: 10.1039/C6CC09916E

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