Fluorogenic probes to monitor cytosolic phospholipase A2 activity

Cheng Yang Ng; Timothy Xiong Wei Kwok; Francis Chee Kuan Tan; Chian-Ming Low; Yulin Lam

doi:10.1039/C6CC09305A

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Fluorogenic probes to monitor cytosolic phospholipase A₂ activity†

Cheng Yang Ng,^a Timothy Xiong Wei Kwok,^a Francis Chee Kuan Tan,

^b Chian-Ming Low*^bc and Yulin Lam*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
E-mail: chmlamyl@nus.edu.sg

^b Department of Anaesthesia, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore

^c Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, MD3 #04-01V, 16 Medical Drive, Singapore 117600, Singapore
E-mail: phclowcm@nus.edu.sg

Abstract

Arachidonic acid derivatives equipped with either one or two fluorescent groups attached to the tip of the alkyl chains were synthesized and shown to function as inhibitor and substrate probes of cPLA₂. The inhibitor probe was demonstrated to perform dual functions of inhibition and imaging while the substrate probe could be used for activity assay.

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Article information

DOI: https://doi.org/10.1039/C6CC09305A
Article type: Communication
Submitted: 22 Nov 2016
Accepted: 09 Jan 2017
First published: 17 Jan 2017

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Chem. Commun., 2017,53, 1813-1816

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Fluorogenic probes to monitor cytosolic phospholipase A₂ activity

C. Y. Ng, T. X. W. Kwok, F. C. K. Tan, C. Low and Y. Lam, Chem. Commun., 2017, 53, 1813 DOI: 10.1039/C6CC09305A

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