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Uptake and transcytosis of functionalized superparamagnetic iron oxide nanoparticles in an in vitro blood brain barrier model

Abstract

Two major hurdles in nanomedicine are the limited strategies for synthesizing stealth nanoparticles and poor efficacy of the nanoparticles in translocating across the blood brain barrier (BBB). Here we examined the uptake and transcytosis of iron oxide nanoparticles (IONPs) grafted with biomimetic phosphorylcholine (PC) brushes in an in vitro BBB model system, and compared it with bare, PEG or PC-PEG mixture grafted IONPs. Hyperspectral imaging indicated IONP co-localization with cells. Quantitative analysis with total reflection X-ray fluorescence spectrometry showed that after 24 h, 78% of PC grafted, 68-69% of PEG or PC-PEG grafted, and 30% of bare IONPs were taken up by the BBB. Transcytosis of IONPs was time-dependent and after 24 h, 16-17% of PC or PC-PEG mixture grafted IONPs had passed the model BBB, significantly more than PEG grafted or bare IONPs. These findings point to PC as a viable grafting strategy for the uptake and transcytosis of nanoparticles.

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Publication details

The article was accepted on 06 Dec 2017 and first published on 07 Dec 2017


Article type: Paper
DOI: 10.1039/C7BM01012E
Citation: Biomater. Sci., 2017, Accepted Manuscript
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    Uptake and transcytosis of functionalized superparamagnetic iron oxide nanoparticles in an in vitro blood brain barrier model

    A. Ivask, E. H. Pilkington, T. Blin, A. Kakinen, H. Vija, M. Visnapuu, J. F. Quinn, M. R. Whittaker, R. Qiao, T. P. Davis, P. C. Ke and N. H. Voelcker, Biomater. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7BM01012E

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