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NOVEL FIBROUS COLLAGEN-BASED CREAM ACCELERATES FIBROBLASTS GROWTH FOR WOUND HEALING APPLICATIONS: IN VITRO AND IN VIVO EVALUATION

Abstract

The present work narrates the development of a novel film forming bovine collagenous cream (BCC) based on bovine collagen (BC). In this study, collagen was isolated from bovine forestomach tissue, a novel source, and prepared cream formulation using some other bioactive ingredients. The electrophoretic pattern of the BC was found to be similar to type I collagen. The purity of BC was examined by Amino acid analysis that confirms the presence of atelo-collagen. The physicochemical properties of BCC such as rheology, spreadability, and temperature stability were characterized. The antimicrobial activity examined against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli and BCC displayed excellent inhibitory effect. In vitro biocompatibility studies using NIH 3T3 fibroblast cells showed enhanced cell viability. FACS analysis revealed non-toxicity nature of BCC with cells. The cell morphology and proliferation on BCC matrix studied using SEM and fluorescence microscopy. In vivo wound healing efficacy of the BCC as a topical wound dressing was demonstrated on full thickness excision wounds in rat models. Healing profile showed that the BCC significantly enhanced re-epithelialization, collagen deposition, and contraction in the wound healing process. The findings of this study provide a newer opportunity for the utilization of the untapped byproduct of meat industry for valorization. We expect that this kind of topical healing cream would be a potential candidate in wound management and the future clinical need.

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Publication details

The article was received on 12 Apr 2017, accepted on 15 Jun 2017 and first published on 19 Jun 2017


Article type: Paper
DOI: 10.1039/C7BM00331E
Citation: Biomater. Sci., 2017, Accepted Manuscript
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    NOVEL FIBROUS COLLAGEN-BASED CREAM ACCELERATES FIBROBLASTS GROWTH FOR WOUND HEALING APPLICATIONS: IN VITRO AND IN VIVO EVALUATION

    U. K. K. Sivalingam, G. Shankar, S. Sampath and R. Chellan, Biomater. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7BM00331E

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