Dual-modal imaging guided highly efficient photothermal therapy using heptamethine cyanine-conjugated hyaluronic acid micelles
Targeted phototherapy and multi-modal imaging in theranostic caneffectively improve the therapeutic efficacy and reduce treatment side effects. Herein, we constructed the novel biocompatible cyanine dye IR808-conjugated hyaluronic acid nanoparticles (HAIR NPs) for photothermal therapy (PTT) with near-infrared fluorescence (FL) and photoacoustic (PA) dual-modal imaging.The nanoparticles showed stable nano-structure in aqueous condition with uniform size distribution. The HAIR NPs were rapidly taken up to human lung cancer cells A549 via CD44 (the hyaluronic acid receptor on the surface of tumor cells) receptor mediated endocytosis. Upon laser irradiation, the HAIR NPs realized good near-infrared fluorescence imaging and photoacoustic imaging in the tumor bearing mice. In addition,The tight nano-structure that come from the covalent link between HA and IR808 could significantly improve light-thermal conversion efficiency of IR808. Under low dose of laser power, the HAIR NPs presented more effective photothermal therapy to suppression of tumor growth than free IR808 in vitro and in vivo. Overall, these results indicate that the HAIR NPs can be considered as an extremely promising nanoplatform in cancer theranostic for targeted PTT under FL and PA dual-modal imaging.