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Bilayered nanofibrous 3D hierarchy as skin rudiment by emulsion electrospinning for burn wound management

Abstract

Mimicking skin extracellular matrix hierarchy, the present work aims to develop a bilayer skin graft comprising a porous cotton-wool-like 3D layer with membranous structure of PCL-chitosan nanofibers. Emulsion electrospinning with differential stirring period of PCL-chitosan emulsion results in development of bilayer 3D structure with varied morphology. The electrospun membrane has fiber diameter ~ 274 nm and pore size ~ 1.16 µm while fluffy 3D layer has fiber diameter ~ 1.62 µm and pore size ~ 62 µm. The 3D layer was further coated with collagen I isolated from Cirrhinus cirrhosus fish scale to improve biofunctionality. Surface coating with collagen I resulted in bundling of fibers together, thereby increasing their average diameter to 2.80 µm and decreasing pore size to ~ 45 µm. The architecture and composition of the scaffold promote efficient cellular activity where interconnected porosity with ECM resembling collagen I coating assists cellular adhesion, infiltration, and proliferation from initial days of fibroblast seeding, while keratinocytes migrate on surface only without infiltrating in the membranous nanofiber layer. Anatomy of the scaffold arising due to variation in pore size distribution at different layers thereby facilitates compartmentalization and prevents initial cellular transmigration. The scaffold also assists in extracellular matrix protein synthesis and keratinocyte stratification in vitro. Further, the scaffold effectively integrates and attaches with third-degree burn wound margin created in rat model and accelerates healing in comparison to standard Tegaderm dressingTM. The bilayer scaffold is thus a promising readily available cost-effective, off-the-shelf matrix as skin substitute.

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Publication details

The article was received on 03 Mar 2017, accepted on 06 Jun 2017 and first published on 13 Jun 2017


Article type: Paper
DOI: 10.1039/C7BM00174F
Citation: Biomater. Sci., 2017, Accepted Manuscript
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    Bilayered nanofibrous 3D hierarchy as skin rudiment by emulsion electrospinning for burn wound management

    P. Pal, P. Dadhich, P. K. Srivas, B. Das, D. Moulik and S. Dhara, Biomater. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7BM00174F

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