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Synchronization of excitable cardiac cultures of different origin

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Abstract

In the present work, we investigated the synchronization of electrical activity in cultured cardiac cells of different origin put in direct contact. In the first set of experiments synchronization was studied in the primary culture cells of neonatal rats taken at different developmental ages, and in the second – in the neonatal rat cardiomyocytes and HL-1 cells. The electrical excitation of cells was recorded using the calcium transient marker Fluor-4. In the confluent cell layers created with the aid of a specially devised mask, the excitation waves and their propagation between areas occupied by cells of different origin were observed. On the level of individual cells, their contact and synchronization was monitored with the aid of scanning fluorescence microscopy. It was found that populations of cultured cells of different origin are able to synchronize, suggesting the formation of electrical coupling between them. The results obtained may be considered as a proof of concept that implanted alien grafted cells are able to create electrical coupling with the host cardiac tissue.

Graphical abstract: Synchronization of excitable cardiac cultures of different origin

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Publication details

The article was received on 02 Mar 2017, accepted on 06 Jun 2017 and first published on 07 Jun 2017


Article type: Paper
DOI: 10.1039/C7BM00171A
Citation: Biomater. Sci., 2017, Advance Article
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    Synchronization of excitable cardiac cultures of different origin

    N. N. Agladze, O. V. Halaidych, V. A. Tsvelaya, T. Bruegmann, C. Kilgus, P. Sasse and K. I. Agladze, Biomater. Sci., 2017, Advance Article , DOI: 10.1039/C7BM00171A

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