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Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells

Abstract

Organized cardiomyocytes alignment is critical to maintain the mechanical properties of the heart. In this study, we present a new and simple strategy to fabricate a biomimetic microchip designed with onion epithelium-like structure and investigate the guided behavior of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) on the substrate. The hiPSC-CMs were observed to be confined by the three dimensional surficial features morphologically, analogous to in vivo microenvironment, and exhibit an organized anisotropic alignment on the onion epithelium-like structure with good beating function. The calcium imaging demonstrated more mature Ca2+ sparks pattern. Furthermore, the expression of sarcomere genes (TNNI3, MYH6 and MYH7), potassium channel genes (KCNE1 and KCNH2), and calcium channel genes (RYR2) were significantly up-regulated on the substrate with onion epithelium-like structure instead of the surface without structure, indicating a more matured status of cardiomyocytes induced by this structure. It appears the biomimetic micropatterned structure, analogous to in vivo, is an important factor that might promote the maturation of hiPSC-CMs, providing new biological insights to guide hiPSC-CMs maturation by biophysical factors. The established approach may offer an effective in vitro model for investigating cardiomyocytes differentiation, maturation and tissue engineering applications.

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Publication details

The article was received on 16 Feb 2017, accepted on 02 Jun 2017 and first published on 12 Jun 2017


Article type: Paper
DOI: 10.1039/C7BM00132K
Citation: Biomater. Sci., 2017, Accepted Manuscript
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    Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells

    C. Xu, L. Wang, Y. Yu, F. Yin, X. Zhang, L. Jiang and J. Qin, Biomater. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7BM00132K

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