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In Vitro and In Vivo Analysis of Visible Light Crosslinkable Gelatin Methacryloyl (GelMA) Hydrogels

Abstract

Photocrosslinkable materials have been frequently used for constructing soft and biomimetic hydrogels for tissue engineering. Although, ultraviolet (UV) light is commonly used for photocrosslinking such materials, its use has been associated with several biosafety concerns such as DNA damage, accelerated aging of tissues, and cancer. Here we report an injectable visible light crosslinked gelatin-based hydrogel for myocardium regeneration. Mechanical characterization revealed that the compressive moduli of the engineered hydrogels could be tuned in the range of 5-56 kPa, by changing the concentrations of the co-initiator and co-monomer in the precursor formulation. In addition, the average pore sizes (26-103 μm) and swelling ratio (7-13%) were also shown to be tunable by varying the hydrogel formulation. In vitro studies showed that visible light crosslinked GelMA hydrogels supported the growth and function of primary cardiomyocytes (CMs). In addition, the engineered materials were shown to be biocompatible in vivo, and could be successfully delivered to the heart after myocardial infarction in an animal model to promote tissue healing. The developed visible light crosslinked hydrogel could be used for the repair of various soft tissues such as the myocardium, and for the treatment of cardiovascular diseases with enhanced therapeutic functionality.

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Publication details

The article was received on 08 Feb 2017, accepted on 04 Jul 2017 and first published on 04 Jul 2017


Article type: Paper
DOI: 10.1039/C7BM00110J
Citation: Biomater. Sci., 2017, Accepted Manuscript
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    In Vitro and In Vivo Analysis of Visible Light Crosslinkable Gelatin Methacryloyl (GelMA) Hydrogels

    I. Noshadi, S. Hong, K. E. Sullivan, E. Shirzaei Sani, R. Portillo Lara, A. Tamayol, S. Shin, A. E. Gao, W. L. Stoppel, L. Black, A. Khademhosseini and N. Annabi, Biomater. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7BM00110J

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