Non-sticky and Antimicrobial Zwitterionic Nanocomposite Dressings for Infected Chronic Wounds
Zwitterionic poly(sulfobetaine acrylamide) (pSBAA)-based nanocomposite hydrogel impregnated with germicidal silver nanoparticles (AgNPs) was synthesized and implemented for treatment of infected chronic wounds. The zwitterionic hydrogels exhibited excellent non-sticky properties and had reinforced mechanical properties by the addition of hectorite nanoclay and poly(ethylene glycol)dimethacrylate as physical and chemical crosslinkers, respectively. In addition, AgNPs were grown within intercalated clay/polymer structure by in situ free radical reduction, as confirmed by UV−vis spectroscopy and transmission electron microscopy (TEM). The silver-contained pSBAA nanocomposite hydrogels (pSBAA/Ag) exhibited germicidal properties against gram-positive S. epidermidis and gram-negative P. aeruginosa. The zwitterionic hydrogels show the higher water content than 2-hydroxyethyl methacrylate (pHEMA) hydrogels, owing to the strong hydration via ionic solvation. The negligible cytotoxicity of pSBAA/Ag hydrogels was assessed with human fibroblasts by the MTT assay. Moreover, the zwitterionic hydrogels demonstrated the excellence resistance to adsorption of bovine serum albumin (BSA). To evaluate the feasibility of clinical application as wound dressing with hydrogels, we created infected diabetic rat models and compared with commercial wound dressings. The results show that pSBAA/Ag hydrogel did not adhered to the newly tissue, and readily removed from the wounds after treatment of 3 days. Moreover, the healing recovery was evaluated by visual observation of infected dorsal wounds on rats with induction of diabetes by Streptozotocin. The finding indicates complete healing with the pSBAA/Ag hydrogels after 15 days, faster than other dressings. The histological examination also proved that the zwitterionic hydrogels facilitated epithelialization and collagen distribution in the infected diabetic wounds. Consequently, the novel non-sticky and antimicrobial zwitterionic nanocomposite hydrogels can have the high potential for the treatment of infected chronic wounds.