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An ultrasensitive electrochemiluminescent D-alanine biosensor based on the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires

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Abstract

Herein, an ultrasensitive and stereo-selective electrochemiluminescent (ECL) biosensor based on ECL signal amplification of luminol by the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires (Au-PtNWs) has been designed for the detection of D-alanine (D-Ala). In the sensor design, D-amino acid oxidase (DAAO) was used to generate the ECL co-reactant (H2O2) for luminol in situ. The reduced graphene oxide-hemin-cysteine (rGO-H-Cys) composite and Au-PtNWs acted not only as amplification labels to amplify signals via synergetic catalysis, but also as an ideal nanocarrier to accelerate electron transfer. A great difference in ECL intensities toward D-Ala and L-Ala was obtained, and an obviously higher intensity was seen for D-Ala. Under optimal conditions, the ECL intensity had a linear relationship with the logarithm of the D-Ala concentration in the range from 1.0 × 10−9 to 5.0 × 10−3 M with a detection limit of 3.3 × 10−10 M (S/N = 3). This study provides a new method for the determination of D-Ala in food with high sensitivity, excellent selectivity, and good stability and reproducibility.

Graphical abstract: An ultrasensitive electrochemiluminescent d-alanine biosensor based on the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires

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Publication details

The article was received on 22 Oct 2017, accepted on 24 Nov 2017 and first published on 24 Nov 2017


Article type: Paper
DOI: 10.1039/C7AY02483E
Citation: Anal. Methods, 2018, Advance Article
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    An ultrasensitive electrochemiluminescent D-alanine biosensor based on the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires

    X. Lin, S. Zhu, Q. Xia, J. Ma and Y. Fu, Anal. Methods, 2018, Advance Article , DOI: 10.1039/C7AY02483E

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