Issue 45, 2017

Fast and simple method for identification of adulteration of cow's milk with urea using diffuse reflectance spectroscopy

Abstract

This work describes the development and application of a simple, fast, cheap, and clean method for the detection of adulteration of raw cow's milk by urea, using a combined spot test/diffuse reflectance spectroscopy procedure. The proposed method is based on the reaction of urea with a chromogenic reagent, p-dimethylaminobenzaldehyde (p-DAB) in acidified methanol, yielding a colored compound on the surface of a filter paper. The reaction parameters were optimized using chemometric experimental design. Reflectometric measurements of the colored product were carried out at 440 nm. The linear range was from 25 to 750 mg L−1 (R = 0.992). The proposed method was successfully applied in the analysis of urea in cow's milk samples, demonstrating that it is a reliable alternative method for the screening and detection of urea in cow's milk.

Graphical abstract: Fast and simple method for identification of adulteration of cow's milk with urea using diffuse reflectance spectroscopy

Article information

Article type
Paper
Submitted
04 Oct 2017
Accepted
28 Oct 2017
First published
06 Nov 2017

Anal. Methods, 2017,9, 6357-6364

Fast and simple method for identification of adulteration of cow's milk with urea using diffuse reflectance spectroscopy

P. R. A. B. de Toledo, A. T. Toci, H. R. Pezza and L. Pezza, Anal. Methods, 2017, 9, 6357 DOI: 10.1039/C7AY02354E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements