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The development of a biomimetic enzyme-linked immunosorbent assay based on the molecular imprinting technique for the detection of enrofloxacin in animal-based food

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Abstract

In this study, a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method using molecularly imprinted polymers (MIPs) as artificial antibodies was developed to detect enrofloxacin (ENRO) in animal-based food. The MIPs were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, adsorption kinetic experiments and isothermal adsorption experiments. The results showed that molecularly imprinted polymers were successfully synthesized with a fast adsorption rate and high adsorption. Under the optimum conditions, the sensitivity (IC50) and limit of detection (IC15) of the established BELISA method were 85.7 ng mL−1 and 1.1 ng mL−1, respectively. The method was applied to the determination of ENRO in spiked beef and shrimp samples, and recoveries ranged from 74.4 to 86.8% with relative standard deviations (for n = 3) between 3.3 and 8.0%. The results correlated well with those obtained using ELISA and high performance liquid chromatography methods.

Graphical abstract: The development of a biomimetic enzyme-linked immunosorbent assay based on the molecular imprinting technique for the detection of enrofloxacin in animal-based food

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Publication details

The article was received on 29 Sep 2017, accepted on 06 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7AY02321A
Citation: Anal. Methods, 2017, Advance Article
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    The development of a biomimetic enzyme-linked immunosorbent assay based on the molecular imprinting technique for the detection of enrofloxacin in animal-based food

    J. Wang, Y. Sang, W. Liu, N. Liang and X. Wang, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY02321A

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