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Development of biomimetic enzyme-linked immunosorbent assay based on molecular imprinting technique for the detection of enrofloxacin in animal food

Abstract

In this study, a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method using molecularly imprinted polymers (MIPs) as a bionic antibody was developed to detect enrofloxacin (ENRO) in animal food. The MIPs were characterized by scanning electron micrographs (SEM), fourier transform infrared spectrometer (FT-IR), adsorption kinetics experiment and isothermal adsorptions experiment, respectively. Results showed that molecularly imprinted polymers were successfully synthesized with a fast adsorption rate and high adsorption. Under the optimum conditions, the sensitivity (IC50) and limit of detection (IC15) of the established BELISA method were 85.7 ng/mL and 1.1 ng/mL, respectively. The method was applied to the determination of ENRO in spiked beef and shrimp samples, and recoveries ranged from 74.4 to 86.8% with relative standard deviations (for n=3) between 3.3 and 8.0 %. Results correlated well with that obtained using ELISA and high performance liquid chromatography method.

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Publication details

The article was received on 29 Sep 2017, accepted on 06 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7AY02321A
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Development of biomimetic enzyme-linked immunosorbent assay based on molecular imprinting technique for the detection of enrofloxacin in animal food

    J. Wang, Y. Sang, W. Liu, N. Liang and X. Wang, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY02321A

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