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Selective and low potential electrocatalytic oxidation and sensing of L-cysteine using metal impurity containing carbon black modified electrode

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Abstract

The detection of thiol-containing amino acids, particularly L-cysteine (L-CySH), without any interference from other biochemicals is a challenging research interest in electroanalytical chemistry. Amongst various electrodes, the nanogold modified electrode has been reported to be effective for low potential electrochemical oxidation (∼0 V vs. Ag/AgCl) and sensing of L-CySH. Herein, we report a conductive carbon black (CB, CL-08) modified glassy carbon electrode (GCE/CL-08) with a high surface area (1000 m2 g−1), prepared by a simple drop-casting technique for efficient electrocatalytic oxidation at the lowest oxidation potential, −0.13 V vs. Ag/AgCl, and sensing of L-CySH in a pH 6.65 phosphate buffer solution. The trace metal impurities such as Ni (0.18 wt%) and Fe (0.42 wt%) in CL-08 are found to be key for such unique and unusual electrocatalytic property observed in this study. Compared with the multiwalled carbon nanotube and graphite powder modified electrodes, the GCE/CL-08 showed about 400 mV reduction in the oxidation potential and twenty times enhancement in the current signal for the CySH. A chronoamperometry detection of L-CySH on the GCE/CL-08 at an applied potential = −0.13 V vs. Ag/AgCl yielded a current linearity from 50 to 700 μM with a LOD = 45.87 nM. There are no interferences by common biochemicals such as ascorbic acid, dopamine, uric acid, xanthine, hypoxanthine and homo-cysteine on the L-CySH oxidation potential. Selective chronoamperometric detection of L-CySH in the blood serum demonstrated ∼100% recovery value as a validation for the present protocol.

Graphical abstract: Selective and low potential electrocatalytic oxidation and sensing of l-cysteine using metal impurity containing carbon black modified electrode

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Publication details

The article was received on 19 Sep 2017, accepted on 11 Nov 2017 and first published on 16 Nov 2017


Article type: Paper
DOI: 10.1039/C7AY02251D
Citation: Anal. Methods, 2017, Advance Article
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    Selective and low potential electrocatalytic oxidation and sensing of L-cysteine using metal impurity containing carbon black modified electrode

    S. Sornambikai, M. R. Abdul Kadir, A. S. Kumar, N. Ponpandian and C. Viswanathan, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY02251D

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