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An Ultrasensitive Sandwich Immunoassay with Glucometer Readout for Portable and Quantitative Detection of Cronobacter Sakazakii

Abstract

Developing portable and low-cost methods for quantitative detection of Cronobacter sakazakii (C. sakazakii) from powdered infant formula plays a significant role in controlling and preventing a range of serious neonatal infections. Here we report a novel methodology for C. sakazakii detection using low-cost, simple, and widely accessible personal glucose meter (PGM) as readout. The electrochemical immunoassay strategy was based on antibodies and glucose oxidase coated silica nanoparticles (SiNP-GOX-IgG) and antibodies functionalized silica-coated magnetic nanoparticles (MNP-IgG). In the process of detecting positive sample, a sandwich immunocomplex was formed between the MNP-IgG, C. sakazakii and SiNP-GOX-IgG. Then the above sandwich composites were dispersed in glucose solution, and the concentration of glucose was measured using PGM. Under optimal conditions, a linear relationship between the decrease of glucose concentration and the logarithm of C. sakazakii concentration was obtained in the concentration range from 9.0×102 to 9.0×107 CFU·mL-1 with a detection limit of 4.2×101 CFU·mL-1 (S/N=3). This simple immunoassay holds great potential as efficient, sensitive and selective and can be used as one of the point-of-care testing (POCT) tool for C. sakazakii detection.

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Publication details

The article was received on 16 Sep 2017, accepted on 11 Oct 2017 and first published on 11 Oct 2017


Article type: Paper
DOI: 10.1039/C7AY02222K
Citation: Anal. Methods, 2017, Accepted Manuscript
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    An Ultrasensitive Sandwich Immunoassay with Glucometer Readout for Portable and Quantitative Detection of Cronobacter Sakazakii

    L. Ye, G. Zhao and W. Dou, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY02222K

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