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Issue 45, 2017
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A highly selective and sensitive fluorescence sensor for the detection of apigenin based on nitrogen doped carbon dots and its application in cell imaging

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Abstract

A highly selective and sensitive fluorescence detection platform based on nitrogen doped carbon dots (N-CDs) is developed for the first time for the determination of apigenin (Api) in celery. N-CDs were obtained by one-step hydrothermal treatment of sunflower seeds. High-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, UV-vis absorption and fluorescence spectroscopy were used to characterize the resultant N-CDs. The presence of Api could lead to the obvious fluorescence quenching. The possible fluorescence quenching mechanism was verified to be a dynamic quenching mechanism. The proposed sensing platform was successfully used to detect Api and exhibited high sensitivity and selectivity. The linearity ranged from 0.1 to 60 μmol L−1 with a detection limit (3σ) of 80 nM. The recovery of this assay was in the range of 90.67–109.02%. Moreover, possessing fascinating luminescence properties, low toxicity and good biocompatibility, the resultant N-CDs have displayed potential applications in cell imaging.

Graphical abstract: A highly selective and sensitive fluorescence sensor for the detection of apigenin based on nitrogen doped carbon dots and its application in cell imaging

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Publication details

The article was received on 14 Sep 2017, accepted on 14 Oct 2017 and first published on 16 Oct 2017


Article type: Paper
DOI: 10.1039/C7AY02202F
Citation: Anal. Methods, 2017,9, 6379-6385
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    A highly selective and sensitive fluorescence sensor for the detection of apigenin based on nitrogen doped carbon dots and its application in cell imaging

    J. Li, J. Song, X. Liang, Q. Ma, L. Shen, Y. Guo and F. Feng, Anal. Methods, 2017, 9, 6379
    DOI: 10.1039/C7AY02202F

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