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Sensitive and reliable paper-based glucose sensing mechanisms with smartphone readout using the L*a*b* color space

Abstract

Paper-based assay platforms play an important role among the rapid test methods. It is essential to combine these promising platforms with smartphones which make on-site analysis possible without using external image processors. In this study, performance of paper-based glucose assay methods was investigated by using L*a*b* color space as quantitative measurement. For this purpose, two glucose assay methods, namely quinone and iodine, were compared in terms of linearity, limit of detection (LOD) and reliability. Lower LOD and higher sensitivity were obtained by the quinone method while the iodine method may lead to large indeterminate errors due to the formation of non-uniform color distribution. Moreover, color measurement strategies were tested by both computer software and mobile applications. For this purpose, scanned images were processed on computer and the results were compared with smartphone based color measurements. Lowest LOD value of 0.38 mM glucose was obtained with the mobile application running on Android operating system. Finally, glucose assay was performed in sour cherry nectar and honey and the results were compared with the spectrophotometric glucose assay. Using smartphones and L*a*b* color space appears to be promising for reliable paper-based sensing as being totally portable.

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Supplementary files

Publication details

The article was received on 04 Sep 2017, accepted on 07 Nov 2017 and first published on 08 Nov 2017


Article type: Paper
DOI: 10.1039/C7AY02124K
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Sensitive and reliable paper-based glucose sensing mechanisms with smartphone readout using the L*a*b* color space

    D. BAŞ, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY02124K

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