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Quantitative and rapid detection of microcystin-LR using time-resolved fluorescence immunochromatographic assay based on europium nanospheres

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Abstract

In the present study, a novel time-resolved fluorescence immunochromatographic assay was established for the rapid quantitative detection of microcystin-leucine arginine (MC-LR). In this method, the europium nanoshpere labelled with anti-MC-LR antibodies was used as the luminescent tracer, dissolved in the running buffer and then added with the sample solution on the pad. MC-LR-BSA and goat anti-mouse antibody were dispensed on the nitrocellulose membrane for the test and the control line, respectively. The optimal parameters were 0.05 g L−1 MC-LR-BSA, 1 : 100 colloidal europium–antibody conjugate, and 10 min reaction time. The linear working range for MC-LR was 0.1–5 μg L−1 with an IC50 of 0.78 μg L−1 and a sensitivity of 0.035 μg L−1. The low cross-reactivity was observed with MC-YR and MC-LF. The assay accuracy was confirmed by the HPLC method with a correlation coefficient of 0.99. When the variable coefficients were 4.4% and 5.4%, the average recoveries of tap and lake water were 94.6% and 102.8%, respectively. The time-resolved fluorescence immunochromatographic assay provides a sensitive, simple, and speedy performance for MC-LR quantitative determination and has a potential use for water sample screening.

Graphical abstract: Quantitative and rapid detection of microcystin-LR using time-resolved fluorescence immunochromatographic assay based on europium nanospheres

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Publication details

The article was received on 24 Aug 2017, accepted on 24 Oct 2017 and first published on 25 Oct 2017


Article type: Paper
DOI: 10.1039/C7AY02036H
Citation: Anal. Methods, 2017, Advance Article
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    Quantitative and rapid detection of microcystin-LR using time-resolved fluorescence immunochromatographic assay based on europium nanospheres

    Y. Zhang, X. Ding, M. Guo, T. Han, Z. Huang, H. Shang and B. Huang, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY02036H

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