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An effective signal enhancement strategy for sensing cysteine based on aluminium-initiated emission

Abstract

An efficient signal enhancement strategy for the detection of cysteine(Cys) was developed, which is based on the combination of Cys-reduced reaction with the chelation of Al3+ with the Cys-promoted product. The Cys probes S-DPM were prepared by adding a 2,4-dinitrobenzenesulfonyl group onto 2’-hydroxyldihydropyrimidobenzimidazole derivative(DPM). Upon treatment with Cys, S-DPM was reduced and released the hydroxyl group in the product DPM, thereby enabling excited-state intramolecular proton transfer process(ESIPT). When Al3+ ions were added, the fluorescence signal was dramatically increased owing to the transformation of the DPM into DPM-Al3+ complexes with very high fluorescence quantum yield(0.865). The proposed method allows the analysis of Cys by modulating the switch of the fluorescence of S-DPM with Cys ranging from 0.01 to 4.5 μM. The detection limit is 17 nM. As expected,the probes S-DPM were successfully applied for the determination of Cys in human serum and plasma samples with recoveries ranging from 97.2.0% to 105.5%. Fluorescence imaging experiments showed that S-DPM can track Cys in living cells. ESIPT compounds are frequently used for the construction of Cys probes, but this method measure the fluorescence of only a part of the Cys-reduced products. The use of S-DPM coupled with Al3+ overcomes this drawback.

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Publication details

The article was received on 23 Aug 2017, accepted on 28 Sep 2017 and first published on 29 Sep 2017


Article type: Paper
DOI: 10.1039/C7AY02026K
Citation: Anal. Methods, 2017, Accepted Manuscript
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    An effective signal enhancement strategy for sensing cysteine based on aluminium-initiated emission

    Z. Wu , X. Chen, H. Cheng , T. Gu, X. Jiaoyun, C. Can and F. C. Gong, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY02026K

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