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Hyaluronan-tyrosine-gold nanoparticle as an enzyme-free colorimetric probe for the detection of phosphorothiolate pesticides

Abstract

Detection of organophosphorus pesticides (OPP) is crucial for food safety and environment protection. Here, we propose an enzyme-free strategy for the detection of phosphorothiolate pesticides, a major class of OPP with P-S bond, by using hyaluronan-tyrosine-gold nanoparticle (HA-Tyr-AuNP) as the colorimetric probe. The strategy is based on the concept that the surface of AuNP can be directly modified by the hydrolyzate of the phosphorothiolate in the strong basic HA-Tyr-AuNP colloidal solution, which in turn affects the aggregation state of AuNP and hence red-to-blue color change. This method is very simple in design and easy to operate. The detection requires only the addition of the analyte into the HA-Tyr-AuNP colloidal solution at room temperature. By virtue of the absorbance variations of the analytical system, the limit of detection for phorate is as low as 0.005 μg mL-1 (i.e., 0.006 mg kg-1), which is lower than the maximum residue limits (MRLs) allowed by the European Union. More importantly, phorate residues in real samples can be directly detected with this method.

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Publication details

The article was received on 15 Aug 2017, accepted on 04 Oct 2017 and first published on 05 Oct 2017


Article type: Paper
DOI: 10.1039/C7AY01969F
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Hyaluronan-tyrosine-gold nanoparticle as an enzyme-free colorimetric probe for the detection of phosphorothiolate pesticides

    L. Yang, X. Zhang, H. Li and L. Jang, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01969F

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