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PH-sensing fluorescence oligonucleotide probes based on an i-motif scaffold: A review.

Abstract

Fluorescence-based approaches have found widespread applications in cell biology. The pH-sensing using fluorescent i-motifs is a potentially useful strategy for real-time, in vivo monitoring of important cellular processes. I-motif scaffold can serve as the recognition element for H+ and is highly programmable, which leads to probes that exhibited different dynamic pH range and possessed slightly different transition midpoint. Moreover, the various methodology can be employed to obtain fluorescence signal, such as incorporating fluorescent cytosine analogues, excimer or FRET labeling. The alternative label-free strategy for i-motif formation and as a consequence, for pH monitoring, is also presented within this review. The strategies used for internalization of i-motif sensors are cited as well as an example of anchoring it into cell membrane for extracellular measurements is described. In this review, we have focused on the recent developments in pH-sensing fluorescence oligonucleotide probes based on an i-motif scaffold, especially in context of intracellular sensing and bioimaging.

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Publication details

The article was received on 09 Aug 2017, accepted on 28 Sep 2017 and first published on 28 Sep 2017


Article type: Critical Review
DOI: 10.1039/C7AY01942D
Citation: Anal. Methods, 2017, Accepted Manuscript
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    PH-sensing fluorescence oligonucleotide probes based on an i-motif scaffold: A review.

    A. Dembska, P. Bielecka and B. Juskowiak, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01942D

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