Jump to main content
Jump to site search

Issue 43, 2017
Previous Article Next Article

pH-Sensing fluorescence oligonucleotide probes based on an i-motif scaffold: a review

Author affiliations

Abstract

Fluorescence-based approaches have found widespread applications in cell biology. pH-Sensing using fluorescent i-motifs is a potentially useful strategy for real-time, in vivo monitoring of important cellular processes. i-Motif scaffolds can serve as the recognition element for H+ and are highly programmable, which leads to probes that exhibit different dynamic pH ranges and possess slightly different transition midpoints. Moreover, various methodologies can be employed to obtain fluorescence signals, such as incorporation of fluorescent cytosine analogues and excimers or FRET labeling. An alternative label-free strategy for i-motif formation and as a consequence, for pH monitoring, is also presented within this review. Strategies used for the internalization of i-motif sensors are cited as well as an example of anchoring them to a cell membrane for extracellular measurements is described. In this review, we have focused on recent developments in pH-sensing fluorescence oligonucleotide probes based on an i-motif scaffold, especially in the context of intracellular sensing and bioimaging.

Graphical abstract: pH-Sensing fluorescence oligonucleotide probes based on an i-motif scaffold: a review

Back to tab navigation

Publication details

The article was received on 09 Aug 2017, accepted on 28 Sep 2017 and first published on 28 Sep 2017


Article type: Critical Review
DOI: 10.1039/C7AY01942D
Citation: Anal. Methods, 2017,9, 6092-6106
  •   Request permissions

    pH-Sensing fluorescence oligonucleotide probes based on an i-motif scaffold: a review

    A. Dembska, P. Bielecka and B. Juskowiak, Anal. Methods, 2017, 9, 6092
    DOI: 10.1039/C7AY01942D

Search articles by author

Spotlight

Advertisements