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Issue 40, 2017
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Ligand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry

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Abstract

A nanodisc is designed as a vehicle to contain GPCRs in a native-like phospholipid environment. It maintains GPCRs in a better thermostability and homogeneity state in a detergent-free buffer compared to a detergent micelle environment. Here, we established an affinity mass spectrometry-based approach to assay the interaction between the adenosine A2A receptor in the nanodisc and eight known ligands. The affinity mass spectrometry results are consistent with the previously published data for binding affinities. The combination of nanodisc and affinity MS techniques allows the identification of the GPCR ligands in a relatively stable, unliganded/apo and native state.

Graphical abstract: Ligand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry

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Publication details

The article was received on 03 Aug 2017, accepted on 18 Sep 2017 and first published on 12 Oct 2017


Article type: Paper
DOI: 10.1039/C7AY01891F
Citation: Anal. Methods, 2017,9, 5851-5858
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    Ligand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry

    J. Ma, Y. Lu, D. Wu, Y. Peng, W. Loa-Kum-Cheung, C. Peng, R. J. Quinn, W. Shui and Z. Liu, Anal. Methods, 2017, 9, 5851
    DOI: 10.1039/C7AY01891F

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