Jump to main content
Jump to site search


A one-step rapid screening test of Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay

Author affiliations

Abstract

A rapid and specific, hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection of Listeria monocytogenes in food and food products, using a real-time turbidimeter platform (LAMP-turbidity). The principle behind this method relies on an increase in a DNA yield, which correlates with the production of magnesium pyrophosphate, and the results can be determined via an amplification curve within 1 h. The specificity test revealed that L. monocytogenes (DMST 17303) was observed from 34.1 to 38.3 min, while thirty strains of non-L. monocytogenes demonstrated no cross-reactions. The limits of detection for purified genomic DNA and pure culture were 800 pg μL−1 and 2.82 × 103 CFU mL−1, respectively. Investigation on 200 raw chicken meat samples indicated that the specificity, sensitivity, and accuracy of LAMP-turbidity were 100%, 62.75%, and 90.50%, respectively. These data suggest that an hly-based, real-time, quantitative LAMP-turbidity assay can be an applicable tool for the epidemiological screening of L. monocytogenes in food and food products.

Graphical abstract: A one-step rapid screening test of Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay

Back to tab navigation

Publication details

The article was received on 18 Jul 2017, accepted on 17 Oct 2017 and first published on 09 Nov 2017


Article type: Paper
DOI: 10.1039/C7AY01750B
Citation: Anal. Methods, 2017, Advance Article
  •   Request permissions

    A one-step rapid screening test of Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay

    S. Wachiralurpan, T. Sriyapai, S. Areekit, P. Sriyapai, D. Thongphueak, S. Santiwatanakul and K. Chansiri, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY01750B

Search articles by author

Spotlight

Advertisements