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Determination of Malachite Green, Crystal Violet, Brilliant Green and Methylene Blue with Micro-Cloud-Point Extraction and Nonlinear Laser Wave-Mixing Detection Interfaced to Micellar Capillary Electrophoresis

Abstract

Laser wave-mixing CE detection method is reported for separation and detection of malachite green (MG), crystal violet (CV), brilliant green (BG), methylene blue (MB), and the leuco-metabolites of MG and CV residues in aquacultures. This method applies a non-ionic surfactant for micro-cloud-point extraction (MCPE) followed by non-aqueous micellar capillary electrophoresis (MCE) for separation and nonlinear laser wave-mixing detection. The log-log plot of the MCE wave-mixing intensity vs. analyte concentration showed a good quadratic dependence with correlation coefficients greater than 0.989. The mass detection limits at S/N of 2 were determined to be 2.27, 1.50, 23.8, 190, 27.61 and 22.2 attogram for MG, CV, BG, MB, leuco-MG, and leuco-CV, respectively. The concentration limits of detection (LOD) at S/N of 2 were determined to be 4.13, 2.72, 10.2, 81.6, 50.2 and 40.3 pg/mL for MG, CV, BG, MB, leuco-MG and leuco-CV, respectively. The intra-day variability (RSD) for the retention times ranges from 0.6 to 1.0% and the average inter-day variability (RSD) ranges from 0.7 to 4.7%. This method is simple, rapid and reliable, and it allows separation and detection of MG, CV, MB, BG, and the leuco-metabolites of MG and CV.

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Publication details

The article was received on 12 Jul 2017, accepted on 29 Aug 2017 and first published on 30 Aug 2017


Article type: Paper
DOI: 10.1039/C7AY01706E
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Determination of Malachite Green, Crystal Violet, Brilliant Green and Methylene Blue with Micro-Cloud-Point Extraction and Nonlinear Laser Wave-Mixing Detection Interfaced to Micellar Capillary Electrophoresis

    J. S. Pradel and W. G. Tong, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01706E

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