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Impedimetric response study for the efficient detection of breast cancer specific biomarker CA 15-3 using tin oxide thin film based immunoelectrode

Abstract

A novel and sensitive RF magnetron sputtered tin oxide (SnO2) thin film based breast cancer immunosensor that fuses the exquisite sensitivity, rapid determination and specificity with the simple, inexpensive and easy technology has been realized. A SnO2 thin film based biocompatible matrix has been used for immobilization of breast cancer specific antibody CA 15-3 by physical adsorption technique for an efficient detection of breast cancer antigen. The fabricated immunoelectrode (antibody CA 15-3 (Ab)/SnO2/Pt/Ti/glass) has been characterized using scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The optimal equivalent circuit model that matches the impedimetric responses of the immunoelectrode describes three distinct regions: the electrolyte solution resistance (Rs), the double layer capacitance (Cdl) and the specific charge transfer resistance (Rct). The SnO2 thin film based immunosensor results in a decrease in the peak oxidation current in CV and DPV studies due to the stearic hindrance caused by macromolecular antibody. The immunoelectrode (Ab/SnO2/Pt/Ti/glass) exhibits a linear increase in electrochemical impedance response on combining with the specific Antigen CA 15-3, in a wide range of antigen from 50 ng/dL to 700 ng/dL (8 U/mL to 120 U/mL) with a Rct sensitivity of 38.63 (ng/dL)-1, fast response time and a high shelf life of more than 20 weeks.

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Publication details

The article was received on 01 Jul 2017, accepted on 23 Sep 2017 and first published on 25 Sep 2017


Article type: Paper
DOI: 10.1039/C7AY01609C
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Impedimetric response study for the efficient detection of breast cancer specific biomarker CA 15-3 using tin oxide thin film based immunoelectrode

    K. A, M. Tomar and V. Gupta, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01609C

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