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Liquid chromatography-mass spectrometry determination of iloperidone on rat dried blood spots: Application to pharmacokinetics

Abstract

A simple, rapid and highly sensitive liquid chromatography-mass spectrometry method was developed and validated for analysis of iloperidone on rat dried blood spots. The chromatographic separation was achieved on an Agilent RP-18 column (250 x 4.6 mm; 5 µm), using 20 mM ammonium acetate (adjusted to pH 5 with TFA) and acetonitrile (60:40 v/v) as a mobile phase at a flow rate of 1.0 mL/min in an isocratic mode. The analytes were detected in positive electro spray ionization mode and monitored selectively at m/z 427 [M+H]+ for iloperidone and paliperidone used as an internal standard. The mean recovery of iloperidone from dried blood spots was 95.7% with an intra and inter-day precision of 9.6%. The assay was linear over a range of 1-300 ng/mL. The limits of detection and quantification were found to be 0.09 ng/mL and 0.3 ng/mL respectively. Stability studies showed that iloperidone was stable on dried blood spots for 1 month at -20°C. The effect of hematocrit to assess the accuracy of extraction of iloperidone from dried blood spots was evaluated and found to be more than 94%. The developed method was applied to study pharmacokinetics of iloperidone by an oral administration of 5 mg/kg in healthy rats.in an isocratic mode. The analytes were detected in positive electro spray ionization mode and monitored selectively at m/z 427 [M+H]+ for iloperidone and paliperidone used as an internal standard. The mean recovery of iloperidone from DBS was 95.7% with an intra and inter-day precision of 9.6% The assay was linear over a range of 1-300ng/mL. The limits of detection and quantification were found to be 0.09 ng/mL and 0.3ng/mL respectively. Stability studies showed that iloperidone was stable on dried blood spots for 1 month at -20°C. The effect of hematocrit to assess the accuracy of extraction of iloperidone from DBS was evaluated and found to be more than 94%. The developed method was applied to study pharmacokinetics of iloperidone by an oral administration of 5 mg/kg in healthy rats.

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Publication details

The article was received on 29 Jun 2017, accepted on 10 Sep 2017 and first published on 11 Sep 2017


Article type: Paper
DOI: 10.1039/C7AY01589E
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Liquid chromatography-mass spectrometry determination of iloperidone on rat dried blood spots: Application to pharmacokinetics

    N. R. Ramisetti, G. P. Katuri, S. Khalid, K. S. Praneetha, D. Dodda and V. Ciddi , Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01589E

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