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Development of competitive ‘pseudo’-ELISA assay for measurement of cocaine and its metabolites using molecularly imprinted polymer nanoparticles.

Abstract

The analytical test-system for cocaine, benzoylecgonine and norcocaine was developed in ELISA format using molecularly imprinted polymeric nanoparticles (nanoMIPs) as synthetic recognition elements that were produced using solid-phase synthesis approach. The experimental conditions of the assay were optimised using a Box-Behnken experimental design protocol. The detection of free cocaine and its metabolites was performed using a competitive binding assay in the model samples and in blood plasma. There was no cross-reactivity of the developed assay towards paracetamol and caffeine. The developed assay had a picomolar limit of detection of cocaine (LOD = 4.24 pM), which was almost three orders of magnitude lower than the LOD expected from commercial antibody-based ELISA (3.3 nM), other attractive features of a new assay included a long shelf-life, lower economic cost and a short production time. Therefore, it is possible to state that nanoMIPs have the potential to become the recognition elements of choice for the development of a new generation of test-systems and sensors.

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Publication details

The article was received on 21 Jun 2017, accepted on 13 Jul 2017 and first published on 14 Jul 2017


Article type: Paper
DOI: 10.1039/C7AY01523B
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Development of competitive ‘pseudo’-ELISA assay for measurement of cocaine and its metabolites using molecularly imprinted polymer nanoparticles.

    Y. Garcia Almanza, K. Smolinska-Kempisty, E. Pereira, E. Piletska and S. A. Piletsky, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01523B

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