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Ultrasensitive and selective colorimetric detection of acetamiprid pesticide based on the enhanced peroxidase-like activity of gold nanoparticles

Abstract

This paper reports a new colorimetric aptasensor for the detection of acetamiprid pesticides with high sensitivity and selectivity based on the enhanced peroxidase-like activity of gold nanoparticles (AuNPs). The aptasensor employs S-18 aptamer as the target specific recognition molecule and the enhanced peroxidase-like activity of AuNPs as the signal transmit element. In the absence of acetamiprid, S-18 aptamer sequences can adsorb on the surface of AuNPs and enhance their negative charge density because of the attached negatively charged DNA backbone, which will inhibit AuNPs peroxidase catalytic activity to oxidize the substance 2,2’-azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) (ABTS). In the presence of acetamiprid, aptamer sequences can specifically bind with pesticide molecules to form acetamiprid-aptamer complex, which can interact with AuNPs surface through the adsorption energy of nucleobases. Thus, the surface active sites of AuNPs are activated and their catalytic activity is enhanced greatly, which enable AuNPs catalyze ABTS to generate green oxidation products with a characteristic absorption peak at 735 nm. The color variation is relevant to the concentration of acetamiprid, which can be determined by a UV-Vis spectrometer or even by naked eyes. The present colorimetric aptasensor has a dynamic range of 10-160 μg/L with the limit of detection as low as 1.02 μg/L, and high selectivity against other competitive pesticides. Moreover, the proposed aptasensor can accurately and efficiently screen out acetamiprid in the representative actual samples that contain trace level of pesticides, suggesting that it has more advantages than traditional methods in the detection of trace pesticide residues. Such ultrasensitive aptasensor will play prominent part in agricultural products and environmental detection.

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Publication details

The article was received on 10 Jun 2017, accepted on 07 Aug 2017 and first published on 08 Aug 2017


Article type: Paper
DOI: 10.1039/C7AY01451A
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Ultrasensitive and selective colorimetric detection of acetamiprid pesticide based on the enhanced peroxidase-like activity of gold nanoparticles

    W. Yang, Y. Wu, H. Tao, J. Zhao, H. Chen and S. Qiu, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01451A

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