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Preparation of polyacrylic acid surface-crosslinked strong fluorescent polymer nanoparticles and their sensitive in vitro imaging of cancer cells and long-life in vivo imaging of in situ tumors

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Abstract

Fluorescent nanoparticles with a novel structure suitable for detecting cancer cells in vitro and in vivo have been prepared. Firstly, a strong, hydrophilic, branched amino polymer was synthesized, and then it was chemically conjugated as much as possible with fluorescein (FAM) to further synthesize a hydrophobic FAM-branched polymer with strong fluorescence. Fluorescein polymer nanoparticles (FPNPs) were then prepared based on hydrophobic interactions of the as-synthesized FAM-branched polymers, and polyacrylic acids (PAAs) were crosslinked on the surface to cover FPNPs and then to prepare PAA surface-crosslinked FPNPs (paa-FPNPs). The as-prepared paa-FPNPs nanoparticles have a fluorescent “core-cover-type” rigid structure and a non-fluorescent flexible structure consisting of the residual chains of the surface-crosslinked PAAs. The paa-FPNPs have four excellent properties as follows: (1) small particle size, a narrow particle distribution and rich negative surface charge; (2) strong fluorescence resulting from the hydrophobic interaction-based aggregation of a large number of the fluorophores conjugated on the branched amino polymers, and from a thin-cover-based weak fluorescence shielding; (3) good protection of the embedded fluorophores against in vivo enzymatic hydrolysis, owing to the outer cover and the negative residual chains of PAAs; (4) weak steric hindrance of the nanoparticle-labeled aptamer recognizing a huge-size target cell. The prepared paa-FPNPs have both a strong fluorescence and a suitable nanoparticle structure for cell imaging. Based on the ligand aptamer labeled by paa-FPNP, this paper reports the sensitive in vitro imaging of cancer cells and long-life in vivo imaging of in situ tumors.

Graphical abstract: Preparation of polyacrylic acid surface-crosslinked strong fluorescent polymer nanoparticles and their sensitive in vitro imaging of cancer cells and long-life in vivo imaging of in situ tumors

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Publication details

The article was received on 05 Jun 2017, accepted on 23 Jul 2017 and first published on 25 Jul 2017


Article type: Paper
DOI: 10.1039/C7AY01392B
Citation: Anal. Methods, 2017, Advance Article
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    Preparation of polyacrylic acid surface-crosslinked strong fluorescent polymer nanoparticles and their sensitive in vitro imaging of cancer cells and long-life in vivo imaging of in situ tumors

    X. Cai, H. He, H. Ding, X. Chen, T. Wei, T. Song, S. You, H. Xie and C. Min, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY01392B

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