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Modified QuEChERS as a novel sample preparation method for analysis of N-nitrosodiethanolamine in shampoo by high performance liquid chromatography

Abstract

This study sought to develop a novel method for the trace analysis of N-nitrosodiethanolamine (NDELA) in shampoos inspired by a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction, followed by an RP-HPLC method with a water-rich mobile phase. To improve the extraction recovery of NDELA and clean-up efficiency of the shampoo matrix, effective parameters of QuEChERS method including sample amounts, sorbents’ types and amounts, solvents’ types and amounts, as well as salts’ amounts were optimized. The obtained results showed that using primary secondary amine and C18 accompanied by silica as a polar sorbent led to the much cleaner chromatogram with lower baseline drift without significant decreasing in extraction recovery of NDELA. Under optimum conditions, satisfactory extraction recovery of NDELA in shampoo (79 ± 3%) was achieved. The concentration range of calibration curve was 5-500 ng mL-1 with regression coefficients of 0.9993. Furthermore, the method’s limit of detection (LODm) and quantification (LOQm) were 14 and 42 μg kg-1 respectively. The intra- and inter-day accuracy and precision of the proposed method yielded relative recoveries of 98-101% and relative standard deviation of less than 5% respectively. Finally, the developed method was applied to determine the occurrence of NDELA in various shampoo samples, and the obtained results were confirmed using liquid chromatography joint to triple quadrupole mass spectrometry.

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Publication details

The article was received on 02 Jun 2017, accepted on 31 Jul 2017 and first published on 02 Aug 2017


Article type: Paper
DOI: 10.1039/C7AY01378G
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Modified QuEChERS as a novel sample preparation method for analysis of N-nitrosodiethanolamine in shampoo by high performance liquid chromatography

    G. Abedi and Z. Talebpour, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01378G

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