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Issue 31, 2017
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Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

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Abstract

Proteinaceous templates are often immobilized prior to polymerization in molecular imprinting which usually entails the need for digestion as a tool for subsequent template removal. The efficiency of digestion, however, has never been investigated in detail in such a context despite the well-known importance of the template removal step in creating selective binding sites. We have demonstrated that native proteins are often not efficiently cleaved by proteinase K, a highly efficient protease enzyme that can digest even keratin. We have studied and optimized the digestion conditions of a model protein, horseradish peroxidase (HRP), by comparing the obtained fragments to those predicted by in silico digestion. The highest cleaving efficiency was obtained after denaturation of the protein with a surfactant and reduction of its disulphide bridges. The protocol developed with HRP was also tested on avidin and was demonstrated to be applicable for template removal from HRP- or avidin-imprinted polymers.

Graphical abstract: Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

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Publication details

The article was received on 26 May 2017, accepted on 02 Jul 2017 and first published on 03 Jul 2017


Article type: Paper
DOI: 10.1039/C7AY01328K
Citation: Anal. Methods, 2017,9, 4496-4503
  • Open access: Creative Commons BY-NC license
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    Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

    J. Erdőssy, E. Kassa, A. Farkas and V. Horváth, Anal. Methods, 2017, 9, 4496
    DOI: 10.1039/C7AY01328K

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