Jump to main content
Jump to site search


Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

Author affiliations

Abstract

Proteinaceous templates are often immobilized prior to polymerization in molecular imprinting which usually entails the need for digestion as a tool for subsequent template removal. The efficiency of digestion, however, has never been investigated in detail in such a context despite the well-known importance of the template removal step in creating selective binding sites. We have demonstrated that native proteins are often not efficiently cleaved by proteinase K, a highly efficient protease enzyme that can digest even keratin. We have studied and optimized the digestion conditions of a model protein, horseradish peroxidase (HRP), by comparing the obtained fragments to those predicted by in silico digestion. The highest cleaving efficiency was obtained after denaturation of the protein with a surfactant and reduction of its disulphide bridges. The protocol developed with HRP was also tested on avidin and was demonstrated to be applicable for template removal from HRP- or avidin-imprinted polymers.

Graphical abstract: Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

Back to tab navigation

Publication details

The article was received on 26 May 2017, accepted on 02 Jul 2017 and first published on 03 Jul 2017


Article type: Paper
DOI: 10.1039/C7AY01328K
Citation: Anal. Methods, 2017, Advance Article
  •   Request permissions

    Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

    J. Erdőssy, E. Kassa, A. Farkas and V. Horváth, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY01328K

Search articles by author

Spotlight

Advertisements