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Fluorescence detection of vitamin B12 in human plasma and urine samples using silver nanoparticles embedded into chitosan in micellar media

Abstract

Herein, a novel fluorescence method has been developed for the determination of vitamin B12 .The method is based on the interaction of vitamin B12 with silver nanoparticles embedded in chitosan (AgNPs-CH) followed by cloud point extraction using Triton X-100 as the nonionic surfactant followed by fluorescence detection. For fluorimetric detection, different excitation wavelengths were applied and excitation wavelength of 250 nm and emission wavelength of 337 nm were selected. The effect of different variables on the fluorescence intensity such as pH of the sample solution, surfactant concentration, incubation temperature and time, electrolyte concentration and amount of AgNPs-CH was investigated. Under the approved condition, a linear calibration curve was validated in the range of 0.2-15.0 ng mL-1 of vitamin B12. Limit of detection (LOD) and limit of quantification (LOQ) based on three and ten times the standard deviation of the blank was 0.036 and 0.119 ng mL-1, respectively. The relative standard deviation for eight replicate measurements of 1.0 and 7.0 ng mL-1 of vitamin B12 was 2.92 and 2.45%, respectively. This probe was used for vitamin B12 determination in human plasma and urine samples with acceptable results and good recoveries.

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Supplementary files

Publication details

The article was accepted on 04 Jun 2017 and first published on 08 Jun 2017


Article type: Paper
DOI: 10.1039/C7AY01297G
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Fluorescence detection of vitamin B12 in human plasma and urine samples using silver nanoparticles embedded into chitosan in micellar media

    N. Pourreza, R. Mirzajani and J. burromandpiroze, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01297G

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