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Ionic liquid based in-situ solvent formation microextraction followed by on-line phase separation coupled with cold vapor-atomic absorption spectrometry for mercury determination in seafood samples

Abstract

An ionic liquid based in-situ solvent formation microextraction (IL-ISFME) followed by on-line phase separation coupled to cold vapor-atomic absorption spectrometry (CV-AAS) was introduced based on using a homemade in-line filter for phase separation. In this method, low cost and easy to reach PTFE powder, as industrial raw material, was filled in a medical syringe and applied for the separation of hydrophobic ionic liquid containing analyte. The developed methodology is simple and eliminates the application of centrifugation in the phase separation step to overcome some limitations of emulsification techniques. In IL-ISFME an ion-pairing agent was added to the sample solution containing hydrophilic IL. In the following, the fine droplets of hydrophobic IL made of promotion of metathesis reaction was recovered via in line filter. To show the applicability of the proposed method, it was applied for extraction and determination of mercury in seafood samples. The effects of some important parameters were investigated and optimized using central composite design (CCD). Under the optimized conditions, linearity of the method was obtained over a range of 2-200 µg L-1 with determination coefficient (R2) higher than 0.996. The enrichment factor (EF) and absolute recovery (AR%) of the method were achieved 5.7 and 57.3%, respectively. The limits of detection (LOD) and quantification (LOQ) were found to be 0.6 and 2µg L-1 respectively. The intra- and inter-day precisions expressed as relative standard deviations (RSD%, n = 3) were 4.8% and 7.6%, respectively. The relative recoveries (RR%) were obtained in the range of 80 to 90.5%, which corresponding to the relative errors of -20% to -9.5%, respectively, confirming the achievement of satisfactory accuracies by the proposed procedure.

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Publication details

The article was received on 19 May 2017, accepted on 05 Aug 2017 and first published on 07 Aug 2017


Article type: Paper
DOI: 10.1039/C7AY01270E
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Ionic liquid based in-situ solvent formation microextraction followed by on-line phase separation coupled with cold vapor-atomic absorption spectrometry for mercury determination in seafood samples

    S. Molaei, S. Seidi, A. Saleh and V. gholipour, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01270E

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