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Glutathione-S-Transferase catalyzed reaction of glutathione for electrochemical biosensing of Temephos, Fenobucarb and Dimethoate

Abstract

This manuscript describes a sensitive bio-electrochemical detection method for extensively used toxic organophosphate pesticides temephos and dimethoate, and organocarbamate fenbucarb, by employing simple mediatorless cyclic voltammetric technique. The sensing scheme is based on the inhibition of glutathione-s-transferase catalytic activity by these pesticides during the course of the conjugation reaction between reduced glutathione and 1-chloro-2,4-dinitrobenzene that results in fall in oxidation peak current of activated glutathione oxidation. Inhibition type of these three pesticides were studied. Fenobucarb showed competitive inhibition with Ki value 10.30 mM, temephos showed non competitive inhibition with Ki value 54.82 mM and dimethoate exhibited a mixed type of inhibition. Calibration curves for all the three pesticides were obtained with detection limits down to 2, 4 and 5 ppb respectively for fenobucarb, temephos, and dimethoate. Method validation was checked with spiked tomato sample following standard solid phase extraction clean - up method. The method is a promising new tool for analysis of water insoluble organophosphate and organocarbamate pesticides.

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Publication details

The article was received on 17 May 2017, accepted on 09 Jun 2017 and first published on 12 Jun 2017


Article type: Paper
DOI: 10.1039/C7AY01258F
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Glutathione-S-Transferase catalyzed reaction of glutathione for electrochemical biosensing of Temephos, Fenobucarb and Dimethoate

    H. BORAH, R. R. Dutta, S. Gogoi, T. Medhi and P. Puzari, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01258F

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