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Issue 27, 2017
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Glutathione-S-transferase-catalyzed reaction of glutathione for electrochemical biosensing of temephos, fenobucarb and dimethoate

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Abstract

This study describes a sensitive bio-electrochemical detection method for extensively used toxic organothiophosphate pesticides temephos and dimethoate, and organocarbamate fenobucarb, by employing a simple mediatorless cyclic voltammetric technique. The sensing scheme is based on the inhibition of the catalytic activity of glutathione-s-transferase using these pesticides during the course of the conjugation reaction between reduced glutathione and 1-chloro-2,4-dinitrobenzene, resulting in a reduction in the oxidation peak current of the activated glutathione oxidation. The types of inhibition of these three pesticides were studied. Fenobucarb showed competitive inhibition with a Ki value of 10.30 mM, temephos showed non-competitive inhibition with a Ki value of 54.82 mM and dimethoate exhibited a mixed type of inhibition. Calibration curves for all the three pesticides were obtained with detection limits down to 2, 4 and 5 ppb for fenobucarb, temephos, and dimethoate, respectively. The method was validated with a spiked tomato sample using the standard solid-phase extraction clean-up method. The method is a promising new tool for analysis of water-insoluble organophosphate and organocarbamate pesticides.

Graphical abstract: Glutathione-S-transferase-catalyzed reaction of glutathione for electrochemical biosensing of temephos, fenobucarb and dimethoate

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Publication details

The article was received on 17 May 2017, accepted on 09 Jun 2017 and first published on 12 Jun 2017


Article type: Paper
DOI: 10.1039/C7AY01258F
Citation: Anal. Methods, 2017,9, 4044-4051
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    Glutathione-S-transferase-catalyzed reaction of glutathione for electrochemical biosensing of temephos, fenobucarb and dimethoate

    H. Borah, R. R. Dutta, S. Gogoi, T. Medhi and P. Puzari, Anal. Methods, 2017, 9, 4044
    DOI: 10.1039/C7AY01258F

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