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Issue 23, 2017
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Imaging acetaldehyde formation during ethanol metabolism in living cells using a hydrazinyl naphthalimide fluorescent probe

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Abstract

The metabolism of ethanol to acetaldehyde has been visualized in living lung epithelial cells using a hydrazinyl naphthalimide fluorescent probe. Utilizing a condensation reaction between carbonyls and a hydrazine moiety, we demonstrate that the fluorescent probe (aldehydefluor-1) AF1 reacts with a range of reactive carbonyl species including formaldehyde, acetaldehyde, glyoxylic acid, and methyl glyoxal. With AF1, it is possible to directly visualize endogenous carbonyl metabolites. Here, we have applied it towards the visualization of acetaldehyde generated from alcohol dehydrogenase mediated ethanol metabolism, validating it as a useful tool to study the roles of alcohol in respiratory disease and other pathological mechanisms.

Graphical abstract: Imaging acetaldehyde formation during ethanol metabolism in living cells using a hydrazinyl naphthalimide fluorescent probe

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Publication details

The article was received on 15 May 2017, accepted on 27 May 2017 and first published on 29 May 2017


Article type: Communication
DOI: 10.1039/C7AY01238A
Citation: Anal. Methods, 2017,9, 3418-3421
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    Imaging acetaldehyde formation during ethanol metabolism in living cells using a hydrazinyl naphthalimide fluorescent probe

    A. G. Reeves, M. Subbarao and A. R. Lippert, Anal. Methods, 2017, 9, 3418
    DOI: 10.1039/C7AY01238A

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