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Issue 24, 2017
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Giant magnetoimpedance based immunoassay for cardiac biomarker myoglobin

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Abstract

Herein, a novel method for the detection of the cardiac biomarker myoglobin (Mb) is proposed by using an integrated giant magnetoimpedance (GMI) based immunosensor. The GMI sensor integrates magnetic sensing elements and a gold film. Micro-patterned GMI sensing elements were prepared from a cobalt-based commercial amorphous ribbon (Metglas® 2714A) using micro-electro-mechanical system (MEMS) technology. The gold film was deposited on the GMI sensing element to act as a support for the immunoplatform. The classical sandwich assay was used for the detection of Mb targeted with Dynabeads by using the antibody–antigen pair combination of biotin–streptavidin. The GMI ratios of the sensors with different concentrations of the antigen against Mb were investigated. As a result, the GMI ratios are significantly improved at high frequency if Mb is present. The sensor has a linear response in the 1 to 10 ng ml−1 concentration range, and the detection limit is 0.5 ng ml−1 at a working frequency of 10 MHz. The method is successfully applied to the determination of Mb, and can be used to detect other cardiac markers like Troponin and CK-MB.

Graphical abstract: Giant magnetoimpedance based immunoassay for cardiac biomarker myoglobin

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Publication details

The article was received on 13 Apr 2017, accepted on 25 May 2017 and first published on 25 May 2017


Article type: Paper
DOI: 10.1039/C7AY00981J
Citation: Anal. Methods, 2017,9, 3636-3642
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    Giant magnetoimpedance based immunoassay for cardiac biomarker myoglobin

    Z. Yang, H. Wang, X. Dong, H. Yan, C. Lei and Y. Luo, Anal. Methods, 2017, 9, 3636
    DOI: 10.1039/C7AY00981J

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