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Isotope dilution quantification of 5-hydroxymethyl-2-furaldehyde in beverages using vortex-assisted liquid–liquid microextraction coupled with ESI-HPLC-MS/MS

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Abstract

Vortex-assisted liquid–liquid microextraction (VALLME) is a fast and simple pretreatment method, which has been applied for the clean-up of beverages containing caramel color. Herein, a reversed-phase C18 column (50 mm × 2.5 mm, 2 μm) was used, and the MS/MS instrument was operated in the multiple reaction monitoring mode for the sensitive and selective detection of 5-hydroxymethyl-2-furaldehyde (HMF). The matrix effect and detection stability were corrected using the isotope dilution method. This method demonstrated low limit of quantification (0.3 μg L−1) and excellent linearity (R2 = 0.999). The recovery rates of HMF ranged from 87.5% to 101.0% in carbonated drinks and from 85.1% to 98.7% in soft drinks. The content of HMF was quantified in thirteen beverage samples, ranging from 0.2 to 14.8 mg L−1. In conclusion, the VALLME coupled with the HPLC-ESI-MS/MS method is a valid and rapid technique for isotope dilution quantification of HMF in beverages.

Graphical abstract: Isotope dilution quantification of 5-hydroxymethyl-2-furaldehyde in beverages using vortex-assisted liquid–liquid microextraction coupled with ESI-HPLC-MS/MS

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Publication details

The article was received on 08 Apr 2017, accepted on 31 May 2017 and first published on 31 May 2017


Article type: Paper
DOI: 10.1039/C7AY00927E
Citation: Anal. Methods, 2017, Advance Article
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    Isotope dilution quantification of 5-hydroxymethyl-2-furaldehyde in beverages using vortex-assisted liquid–liquid microextraction coupled with ESI-HPLC-MS/MS

    T. Feng, X. Liang, J. Wu, L. Qin, M. Tan, B. Zhu and X. Xu, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY00927E

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