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Fast gradient normal-phase high pressure liquid chromatography for rapid baseline separation of nine vitamin E homologues in palm tocotrienol rich fraction (TRF)

Abstract

A rapid, accurate and precise normal-phase high pressure liquid chromatographic method was developed and validated for simultaneous determination of nine vitamin E homologues comprised of α-tocomonoenol, α-, β-, γ-, δ-tocopherols and α-, β-,γ-, δ- tocotrienols in tocotrienol rich fraction (TRF) derived from palm oil. The simultaneous chromatographic separation of vitamin E homologues was performed on a high purity silica (4.6 mm x 150 mm, 3.0 µm) LC column thermostated at 40 °C using n-heptane/ethyl acetate as mobile phase programmed via gradient elution. Detection wavelength was set at 290 nm for photodiode array (PDA) detector. The calibration standard curves were linear over the concentration range from 0.1 – 0.5 mg/mL for all homologues with overall R2 > 0.9980. The overall precision was less than RSD 2.0 % and the recovery ranged between 95 – 105 % for all homologues. The method was selective for all homologues and the separation run was completed in 17 minutes. In conclusion, a method has been successfully developed and validated that is specific for the analysis of vitamin E homologues in palm TRF and suitable for routine quality control for all vitamin E homologues in palm TRF at different purity.

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Publication details

The article was received on 08 Apr 2017, accepted on 06 Aug 2017 and first published on 07 Aug 2017


Article type: Paper
DOI: 10.1039/C7AY00924K
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Fast gradient normal-phase high pressure liquid chromatography for rapid baseline separation of nine vitamin E homologues in palm tocotrienol rich fraction (TRF)

    O. H. Tan, E. H. P. Tan and H. I. Ooi, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY00924K

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