Jump to main content
Jump to site search


Alternative method for lactulose quantification in the presence of lactose in milk using HILIC with refractive index detection

Author affiliations

Abstract

An alternative chromatographic method, HILIC-RID, has been developed for lactulose quantification in the presence of lactose in milk samples. Eighteen commercial UHT milk samples were analyzed. The best separation results were achieved with a mobile phase composed of acetonitrile–water (75 : 25) using an isocratic flow of 1 mL min−1. The limits of detection and quantification were 22.68 ± 8.10 and 75.61 ± 27.01 mg L−1, respectively. Recovery ranged from 98.5 to 103.6% for three lactulose fortification levels. All samples presented low coefficients of variation (0.31–2.16%) for lactulose quantification, indicating good method reproducibility. ANOVA test showed all samples differ from the others at the 99% confidence level and allowed an inference about the lack of uniformity in Brazilian UHT milk. A lactulose amount above 600 mg L−1 found in a specific sample indicated a possible recirculation occurrence during its processing. The results attested the applicability of the HILIC-RID method as an alternative tool for quality control, heat damage investigation and food inspection.

Graphical abstract: Alternative method for lactulose quantification in the presence of lactose in milk using HILIC with refractive index detection

Back to tab navigation

Publication details

The article was received on 04 Apr 2017, accepted on 28 Jun 2017 and first published on 03 Jul 2017


Article type: Paper
DOI: 10.1039/C7AY00890B
Citation: Anal. Methods, 2017, Advance Article
  •   Request permissions

    Alternative method for lactulose quantification in the presence of lactose in milk using HILIC with refractive index detection

    L. Natália de Oliveira Neves, R. Guzella de Carvalho, J. A. Kopke de Aguiar and P. H. Fonseca da Silva, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY00890B

Search articles by author

Spotlight

Advertisements