Issue 19, 2017

An azidocoumarin-based fluorescent probe for imaging lysosomal hydrogen sulfide in living cells

Abstract

A commercially available fluorescent hydrogen sulfide (H2S) probe 7-azido-4-methylcoumarin (AzMC) was developed into its lysosome-targeted counterpart Lyso-C via a four-step synthetic approach. Lyso-C displayed fast response (within 5 min), excellent sensitivity (with a detection limit of 37 nM) and high selectivity toward H2S. More importantly, Lyso-C was successfully applied to imaging lysosomal H2S and showed potential capability to quantitatively detect H2S in living cells.

Graphical abstract: An azidocoumarin-based fluorescent probe for imaging lysosomal hydrogen sulfide in living cells

Supplementary files

Article information

Article type
Paper
Submitted
31 Mar 2017
Accepted
18 Apr 2017
First published
18 Apr 2017

Anal. Methods, 2017,9, 2859-2864

An azidocoumarin-based fluorescent probe for imaging lysosomal hydrogen sulfide in living cells

Q. Xie, W. Liu, X. Liu, F. Ouyang, Y. Kuang and J. Jiang, Anal. Methods, 2017, 9, 2859 DOI: 10.1039/C7AY00862G

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