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Issue 32, 2017
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A simple pre-column derivatization method for the determination of mancozeb technical (fungicide) by reverse phase HPLC-UV

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Abstract

A simple and fast pre-column derivatization high performance liquid chromatographic method for the technical analysis of mancozeb was developed and validated in the present study. The mobile phase consists of a mixture of acetonitrile and 0.1% (v/v) formic acid in the proportion 60 : 40. This was found to give a sharp peak of the S-ethyl derivative of mancozeb (S-ethyl ethylene bis-dithiocarbamate) at a retention time of 9.12 minutes. HPLC analysis of mancozeb was carried out at a wavelength of 272 nm with a flow rate of 1.0 mL per minute. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient of 0.999 in the concentration range of 8.2 mg L−1 to 32.3 mg L−1 for the derivative of mancozeb technical. The method was validated for specificity, linearity, precision and accuracy.

Graphical abstract: A simple pre-column derivatization method for the determination of mancozeb technical (fungicide) by reverse phase HPLC-UV

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Publication details

The article was received on 28 Mar 2017, accepted on 06 Jul 2017 and first published on 02 Aug 2017


Article type: Paper
DOI: 10.1039/C7AY00830A
Citation: Anal. Methods, 2017,9, 4702-4708
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    A simple pre-column derivatization method for the determination of mancozeb technical (fungicide) by reverse phase HPLC-UV

    N. H. Petha, R. S. Lokhande, D. T. Seshadri, Raju M. Patil, T. S. Bhagat and J. G. Patil, Anal. Methods, 2017, 9, 4702
    DOI: 10.1039/C7AY00830A

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