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Stable Isotope Labeling Assisted LC-MS Method for Determination of Polyamines in Micro Tissues of Rice

Abstract

Stable isotope labeling assisted LC-MS method were developed for highly sensitive quantitation of polyamines in roots and leaves of rice plants under cadmium stress. Successful quantitation of polyamines in minute amounts of plant extracts allowed the detection of the spatial and temporal changes in an organ/tissue–specific manner. Accumulations of putrescine under cadmium stress were observed in both the tips of roots and leaves. However, different regulations of spermine contents in the two regions were observed. Meanwhile, the influence of cadmium stress from the medium was found persistent towards the growth of roots after cultivation in 6 days, whereas the leaves gradually reached homeostasis after 3 days. Moreover, our micro-regional analysis on leaf tissues have for the first time revealed that the influences of cadmium stress towards the contents of polyamines gradually translocated directionally on the leaves. Typically, the influences towards the contents of major polyamines shifted from the sheath and central zone towards the tip and peripheral zone.

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Publication details

The article was received on 26 Mar 2017, accepted on 06 May 2017 and first published on 08 May 2017


Article type: Paper
DOI: 10.1039/C7AY00805H
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Stable Isotope Labeling Assisted LC-MS Method for Determination of Polyamines in Micro Tissues of Rice

    Q. Wang, T. Ye, S. Zheng, E. Ye, R. Wang and Y. Feng, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY00805H

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