Jump to main content
Jump to site search


Label-free fluorescence assay for rapid detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates

Author affiliations

Abstract

Ribonuclease H (RNase H), a highly conserved damage-repair protein, hydrolyzes RNA in the DNA:RNA hybrid duplex and breaks RNA/DNA junctions. In this study, we demonstrated a unique Tb3+-based fluorescence assay that is low cost, facile, and label-free for assaying RNase H activity and inhibitions by using a G-quadruplex formation strategy. This novel assay method can detect RNase H at the lowest detection limit of 2 U mL−1 under optimal conditions. We demonstrated the utility of the assay by antibiotics screening and identified ethidium bromide (EB) and Gentamycin as RNase H inhibitors. This approach demonstrated that Tb3+ could be used as a functional tool in specific fields in the future.

Graphical abstract: Label-free fluorescence assay for rapid detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 17 Mar 2017, accepted on 05 May 2017 and first published on 05 May 2017


Article type: Paper
DOI: 10.1039/C7AY00709D
Citation: Anal. Methods, 2017, Advance Article
  •   Request permissions

    Label-free fluorescence assay for rapid detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates

    K. Wu, C. Ma, H. Liu, H. He, W. Zeng and K. Wang, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY00709D

Search articles by author